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The mutants exhibited a significant effect on the nanobodies stability, as evidenced by the Cartesian ddG values ranging from -8.9 kcal/mol to 63.8 kcal/mol for PV2 and 9.4 kcal/mol to 73.3 kcal/mol for PV3

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The changes in glycated hemoglobin, as the primary outcome, in the probiotics+BBR [least-squares mean (95% CI), 1.04 (1.19, 0.89)%] and BBR-alone group [0.99 (1.16, 0.83)%] were significantly greater than that in the placebo and probiotics-alone groups [0.59 (0.75, 0.44)%, 0.53 (0.68, 0.37)%, p 90) to 95 mL/min (eGFR 3044), resulting in 4.4-fold higher plasma concentrations (253)