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In deeply anaesthetised rats (placed in ventral position) the tail was transected with a surgical scalpel 3 cm from the tip (and submerged into a tube with 15 ml of saline at room temperature in vertical position) and the duration and amount of bleeding were measured to evaluate the hemostatic effect of the agents or saline administration

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Eyes were then washed, dehydrated and embedded in paraffin wax

The probe accurately visualizes tumors and metastases in mice, suggesting that it could be a convenient tool for fluorescence-guided cancer surgery (149)