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For anterograde tracing of the PVN DMV circuit, rAAV-hSyn-Cre-mCherry-WPRE-hGH polyA (AAV2/1-hSyn-Cre-mCherry, AAV2/1, 1.03 10 13 vg/mL) virus was injected into the PVN and Cre-dependent rAAV-EF1-DIO-mCherry-WPRE-hGH pA (AAV-DIO-mCherry, AAV2/9, 5.31 10 12 vg/mL) virus was injected into the DMV of C57 mice

367,520 Duloxetine relieves pain by increasing the synaptic availability of serotonin and norepinephrine in descending pathways, enhancing endogenous pain modulation

Compared with unmodified disks carrying plasmid DNA, nanodisks modified with L17E demonstrated enhanced endosomal escape and improved transfection efficiency in cell culture