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In addition, the combination regimen may have a better antifibrotic effect

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Purified samples were washed and eluted in Laemmli buffer with 5% -mercaptoethanol, followed by running lysates on 7.5% SDSPAGE gels followed by transfer to nitrocellulose for Western blot analysis, probing with primary and secondary antibodies directed against proteins of interest, DP and Ect2, with GAPDH as a loading control for whole-cell lysates